Galnt1 Protein (AA 41-559) (His tag)

Catalog No. ABIN6388150

Product Details

Target: Galnt1 (Udp-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 1 (GalNAc-T1) (Galnt1))

Protein Type: Recombinant

Protein Characteristics: AA 41-559

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This Galnt1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: ADPGLPAGDV LELVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSRGQVITF LDAHCECTAG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRLGLRRKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCTGSRSQQ WLLRNVTLPE IFHHHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Galnt1 (Udp-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 1 (GalNAc-T1) (Galnt1))

Alternative Name: Galnt1 (Galnt1 Products)

Synonyms: galnac-t1 Protein, GALNT1 Protein, GALNAC-T1 Protein, polypeptide N-acetylgalactosaminyltransferase 1 L homeolog Protein, polypeptide N-acetylgalactosaminyltransferase 1 Protein, galnt1.L Protein, GALNT1 Protein, galnt1 Protein, LOC100181305 Protein, Galnt1 Protein

Background: Galnt1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. It catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant mouse Galnt1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 60.5kDa (528aa) 50-70KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_038842

UniProt: O08912