SEMA7A Protein (AA 45-648) (hIgG-His-tag)

Catalog No. ABIN6388152

Product Details

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Protein Type: Recombinant

Protein Characteristics: AA 45-648

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SEMA7A protein is labelled with hIgG-His-tag.

Application: SDS-PAGE (SDS)

Sequence: ADPQGHLRSG PRIFAVWKGH VGQDRVDFGQ TEPHTVLFHE PGSSSVWVGG RGKVYLFDFP EGKNASVRTV NIGSTKGSCL DKRDCENYIT LLERRSEGLL ACGTNARHPS CWNLVNGTVV PLGEMRGYAP FSPDENSLVL FEGDEVYSTI RKQEYNGKIP RFRRIRGESE LYTSDTVMQN PQFIKATIVH QDQAYDDKIY YFFREDNPDK NPEAPLNVSR VAQLCRGDQG GESSLSVSKW NTFLKAMLVC SDAATNKNFN RLQDVFLLPD PSGQWRDTRV YGVFSNPWNY SAVCVYSLGD IDKVFRTSSL KGYHSSLPNP RPGKCLPDQQ PIPTETFQVA DRHPEVAQRV EPMGPLKTPL FHSKYHYQKV AVHRMQASHG ETFHVLYLTT DRGTIHKVVE PGEQEHSFAF NIMEIQPFRR AAAIQTMSLD AERRKLYVSS QWEVSQVPLD LCEVYGGGCH GCLMSRDPYC GWDQGRCISI YSSERSVLQS INPAEPHKEC PNPKPDKAPL QKVSLAPNSR YYLSCPMESR HATYSWRHKE NVEQSCEPGH QSPNCILFIE NLTAQQYGHY FCEAQEGSYF REAQHWQLLP EDGIMAEHLL GHACALALEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Alternative Name: SEMA7A (SEMA7A Products)

Synonyms: 2900057C09Rik Protein, CDw108 Protein, H-Sema-L Protein, M-Sema-L Protein, Semal Protein, CD108 Protein, H-SEMA-K1 Protein, JMH Protein, SEMAK1 Protein, SEMAL Protein, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A Protein, semaphorin 7A (John Milton Hagen blood group) Protein, Sema7a Protein, SEMA7A Protein

Background: SEMA7A, also known as semaphorin-7A isoform 1, is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. It promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. It plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. It promotes production of proinflammatory cytokines by monocytes and macrophages. Recombinant human SEMA7A, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 95.7kDa (846aa) 70-100kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_003603

UniProt: O75326

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size