HNMT Protein (AA 1-292) (His tag)
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- Target See all HNMT Proteins
- HNMT (Histamine N-Methyltransferase (HNMT))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-292
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HNMT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C
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Histamine N-Methyltransferase (HNMT) (AA 1-51) protein (GST tag)
HNMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Histamine N-Methyltransferase (HNMT) (AA 184-292) protein (GST tag)HNMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Histamine N-Methyltransferase (HNMT) (AA 1-292) protein (His tag)HNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Histamine N-Methyltransferase (HNMT) (AA 1-292) protein (Strep Tag)Crystallography grade HNMT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Histamine N-Methyltransferase (HNMT) (AA 1-295) protein (Strep Tag)Crystallography grade HNMT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl ( pH 8.0) buffer containing 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- HNMT (Histamine N-Methyltransferase (HNMT))
- Alternative Name
- HNMT (HNMT Products)
- Synonyms
- HMT Protein, HNMT-S1 Protein, HNMT-S2 Protein, hmt Protein, hnmt Protein, hnmt-s1 Protein, hnmt-s2 Protein, zgc:100917 Protein, HNMT Protein, 1500031F01Rik Protein, AI788969 Protein, HMT A Protein, hnmt-a Protein, hnmt-b Protein, histamine N-methyltransferase Protein, histamine N-methyltransferase S homeolog Protein, histamine N-methyltransferase-like Protein, histamine N-methyltransferase L homeolog Protein, HNMT Protein, hnmt.S Protein, hnmt Protein, Hnmt Protein, LOC100228106 Protein, hnmt.L Protein
- Background
- HNMT also known as Histamine N-methyltransferase. HNMT is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT inactivates histamine by N-methylation. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme histamine N-methyltransferase (HNMT) in bronchus, kidney, and the central nervous system. It plays an important role in degrading histamine and in regulating the airway response to histamine. Recombinant human HNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 37.4 kDa (328aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_008826
- UniProt
- P50135
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