NFAT1 Protein (AA 396-678) (His tag)

Catalog No. ABIN6388198

Product Details

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Protein Type: Recombinant

Protein Characteristics: AA 396-678

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This NFAT1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: PLEWPLSSQS GSYELRIEVQ PKPHHRAHYE TEGSRGAVKA PTGGHPVVQL HGYMENKPLG LQIFIGTADE RILKPHAFYQ VHRITGKTVT TTSYEKIVGN TKVLEIPLEP KNNMRATIDC AGILKLRNAD IELRKGETDI GRKNTRVRLV FRVHIPESSG RIVSLQTASN PIECSQRSAH ELPMVERQDT DSCLVYGGQQ MILTGQNFTS ESKVVFTEKT TDGQQIWEME ATVDKDKSQP NMLFVEIPEY RNKHIRTPVK VNFYVINGKR KRSQPQHFTY HPVHHHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 40 % glycerol, 1  mM DTT.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: NFATC2 (NFAT1 Products)

Synonyms: NFATC2 Protein, AI607462 Protein, NF-ATc2 Protein, NFAT1 Protein, NFAT1-D Protein, Nfatp Protein, NFATP Protein, nuclear factor of activated T-cells 2 Protein, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 Protein, NFATC2 Protein, nfatc2 Protein, Nfatc2 Protein

Background: NFATC2, also known as nuclear factor of activated T-cells cytoplasmic 2 isoform C, is a member of the nuclear factor of activated T cells family. This protein plays a vital role in the course of T helper cell activation, differentiation, and effector function. Although KO of an individual NFAT isoform in T cells leads to rather mild effects, T cells deficient for NFATC1 and 2 completely fail to produce T helper cell effector cytokines, such as the interleukins IL-2 and IL-4 or interferon gamma. Also, it is highly phosphorylated and retained in the cytoplasm. Following T cell receptor (TCR) stimulation, dephosphorylation by calcium-activated calcineurin induces a conformational change of NFATC2 that exposes one or more nuclear localization sequences. Recombinant human NFATC2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 33.1kDa (290aa) 28-40kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_775114

UniProt: Q13469

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling