SPARC Protein (His tag)
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- Target See all SPARC Proteins
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Human Cells
- Purification tag / Conjugate
- This SPARC protein is labelled with His tag.
- Sequence
- APQQTEVAEE IVEEETVVEE TGVPVGANPV QVEMGEFEDG AEETVEEVVA DNPCQNHHCK HGKVCELDES NTPMCVCQDP TSCPAPIGEF EKVCSNDNKT FDSSCHFFAT KCTLEGTKKG HKLHLDYIGP CKYIAPCLDS ELTEFPLRMR DWLKNVLVTL YERDEGNNLL TEKQKLRVKK IHENEKRLEA GDHPVELLAR DFEKNYNMYI FPVHWQFGQL DQHPIDGYLS HTELAPLRAP LIPMEHCTTR FFETCDLDND KYIALEEWAG CFGIKEQDIN KDLVIVDHHH HHH
- Characteristics
- This Secreted Protein Acidic and Rich in Cysteineproteins contains the following tag (Position, Tag): C-6His
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SPARC Protein
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) protein (His tag)
SPARC Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) protein (His tag)SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) protein (His tag)SPARC Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-297) protein (His tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) protein (His tag)Crystallography grade SPARC Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) protein (His tag)Crystallography grade SPARC Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
- Alternative Name
- Secreted Protein Acidic and Rich in Cysteine (SPARC Products)
- Synonyms
- ON Protein, AA517111 Protein, BM-40 Protein, hm:zeh0062 Protein, SPARC Protein, sparc Protein, secreted protein acidic and cysteine rich Protein, secreted acidic cysteine rich glycoprotein Protein, secreted protein, acidic, cysteine-rich (osteonectin) Protein, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Protein, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Protein, SPARC Protein, Sparc Protein, sparc Protein, sparc.L Protein, sparc.S Protein
- Molecular Weight
- 33.6 kDa
- UniProt
- P07214
- Pathways
- Autophagy
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