AKR1C1 Protein (AA 1-323) (His tag)

Catalog No. ABIN666955

Product Details

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Protein Type: Recombinant

Protein Characteristics: AA 1-323

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This AKR1C1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: AKR1C1, 1-323aa, Human, His tag, E.coli

Purity: > 90 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Storage: 4 °C

Target Details

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name: AKR1C1 (DDH Products)

Synonyms: 2-ALPHA-HSD Protein, 20-ALPHA-HSD Protein, C9 Protein, DD1 Protein, DD1/DD2 Protein, DDH Protein, DDH1 Protein, H-37 Protein, HAKRC Protein, HBAB Protein, MBAB Protein, aldo-keto reductase family 1 member C1 Protein, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) Protein, AKR1C1 Protein

Background: AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1, Aldo-keto reductase family 1, member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase, 20 alpha hydroxysteroid dehydrogenase, 2ALPHAHSD, AK1C1, MBAB, Aldo keto reductase family 1 member C1, C9, Chlordecone reductase homolog, Chlordecone reductase homolog HAKRC, DD1, Dihydrodiol dehydrogenase 1, Dihydrodiol dehydrogenase 1/2, H37, HAKRC, Hepatic dihydrodiol dehydrogenase, High affinity hepatic bile acid-binding protein, Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase, Type II 3 alpha hydroxysteroid dehydrogenase,. NCBI no.: NP_001344

Molecular Weight: 38.9 kDa (343aa), confirmed by MALDI-TOF.

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process