PNP Protein (AA 1-289) (His tag)

Catalog No. ABIN667267

Product Details

Target: PNP (NP) (Nucleoside Phosphorylase (NP))

Protein Type: Recombinant

Protein Characteristics: AA 1-289

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PNP protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: PNP, 1-289aa, Human, His tag, E.coli

Purity: > 90 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol

Storage: 4 °C

Target Details

Target: PNP (NP) (Nucleoside Phosphorylase (NP))

Alternative Name: PNP (NP Products)

Synonyms: NP Protein, PRO1837 Protein, PUNP Protein, Np Protein, AL024301 Protein, AU015798 Protein, Np-1 Protein, Np-2 Protein, Pnp1 Protein, purine nucleoside phosphorylase Protein, purine-nucleoside phosphorylase Protein, PNP Protein, Pnp Protein

Target Type: Viral Protein

Background: PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2’-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PUNP. NCBI no.: NP_000261

Molecular Weight: 34.2 kDa (309aa) confirmed by MALDI-TOF

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus