Iron-Sulfur Cluster Scaffold Homolog (E. Coli) (ISCU) (AA 35-167) protein (His tag)

Details for Product No. ABIN667303, Supplier: Log in to see
Protein Name
  • nifun
  • zC191D15.3
  • si:ch211-191d15.3
  • 2310020H20Rik
  • HML
  • ISU2
  • NIFU
  • NIFUN
  • hnifU
  • RGD1309562
  • AA407971
  • Nifu
  • Nifun
  • iron-sulfur cluster assembly enzyme ISCU, mitochondrial
  • iron-sulfur cluster assembly enzyme
  • iron-sulfur cluster assembly enzyme a
  • LOC409130
  • ISCU
  • iscua
  • Iscu
Protein Characteristics
AA 35-167
2
1
1
1
1
1
Origin
Human
6
1
1
Source
Escherichia coli (E. coli)
4
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This ISCU protein is labelled with His tag.
Application
SDS-PAGE (SDS)
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Characteristics ISCU, 35-167aa, Human, His tag, E.coli
Purity > 90 % by SDS - PAGE
Protein Name
Background Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Iron-sulfur cluster assembly enzyme ISCU, HML, hnifU, ISU2, NIFU, NIFUN. NCBI no.: NP_998760
Molecular Weight 16.7kDa (154aa), confirmed by MALDI-TOF
Restrictions For Research Use only
Format Liquid
Concentration 0.5mg/ml (determined by Bradford assay)
Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl
Storage 4 °C
Supplier Images
SDS-PAGE (SDS) image for Iron-Sulfur Cluster Scaffold Homolog (E. Coli) (ISCU) (AA 35-167) protein (His tag) (ABIN667303) Iron-Sulfur Cluster Scaffold Homolog (E. Coli) (ISCU) (AA 35-167) protein (His tag)
Background publications Tong, Rouault: "Functions of mitochondrial ISCU and cytosolic ISCU in mammalian iron-sulfur cluster biogenesis and iron homeostasis." in: Cell metabolism, Vol. 3, Issue 3, pp. 199-210, 2006 (PubMed).

Tong, Rouault: "Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells." in: The EMBO journal, Vol. 19, Issue 21, pp. 5692-700, 2000 (PubMed).

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