Dual Specificity Phosphatase 3 (DUSP3) (AA 1-185) (Active) protein (His tag)

Catalog No. ABIN667577

This Recombinant not_set protein is produced in Escherichia coli (E. coli).

Quick Overview for Dual Specificity Phosphatase 3 (DUSP3) (AA 1-185) (Active) protein (His tag) (ABIN667577)

Target: Dual Specificity Phosphatase 3 (DUSP3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-185

Purification tag / Conjugate: His tag

Sequence: MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP

Characteristics: DUSP3, 1-185 aa , Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity is > 1,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 2 mM DTT, 0.15M NaCl.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Dual Specificity Phosphatase 3 (DUSP3)

Alternative Name: VHR/DUSP3

Target Type: Viral Protein

Background: DuSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DuSP3 is expressed in both breast and ovarian tissues. DuSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DuSP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 22.6 kDa (205aa) confirmed by MALDI-TOF

NCBI Accession: NP_004081

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades