PSME3 Protein (PSME3) (AA 1-254) (His tag)
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- Target See all PSME3 Proteins
- PSME3
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-254
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PSME3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- PSME3, 1-254aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PSME3 Protein
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PSME3 (PSME3) (AA 1-267) protein (His tag)
PSME3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
PSME3 (PSME3) (AA 2-254) protein (His tag)PSME3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
PSME3 (PSME3) (AA 1-254) protein (GST tag)PSME3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
PSME3 (PSME3) (AA 2-254) protein (His tag)Crystallography grade PSME3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
PSME3 (PSME3) (AA 2-252), (partial) protein (GST tag)PSME3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
PSME3 (PSME3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PSME3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl
- Storage
- 4 °C
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- Target
- PSME3
- Alternative Name
- PSME3 (PSME3 Products)
- Synonyms
- AA410043 Protein, AU020960 Protein, Ki Protein, PA28gamma Protein, REGgamma Protein, pa28g Protein, Ab2-371 Protein, PA28-gamma Protein, PA28G Protein, REG-GAMMA Protein, psme3b Protein, proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) Protein, proteasome activator subunit 3 Protein, proteasome activator subunit 3 S homeolog Protein, Psme3 Protein, PSME3 Protein, psme3.S Protein
- Background
- PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Proteasome activator complex subunit 3 isoform 1, Ki, PA28-gamma, PA28G, REG-GAMMA. NCBI no.: NP_005780
- Molecular Weight
- 31.7kDa (274aa), confirmed by MALDI-TOF
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA
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