PFKM Protein (AA 1-780) (His tag)
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- Target See all PFKM Proteins
- PFKM (phosphofructokinase, Muscle (PFKM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-780
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PFKM protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- PFKM, 1-780 aa, Human, His-tagged, Recombinant, E.coli
- Purity
- > 80 % by SDS - PAGE
- Top Product
- Discover our top product PFKM Protein
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phosphofructokinase, Muscle (PFKM) (AA 681-780) protein (GST tag)
PFKM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
phosphofructokinase, Muscle (PFKM) protein (GST tag,His tag)PFKM Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
phosphofructokinase, Muscle (PFKM) (AA 2-780) protein (His tag)Crystallography grade PFKM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
phosphofructokinase, Muscle (PFKM) (AA 2-780) protein (His tag)Crystallography grade PFKM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
phosphofructokinase, Muscle (PFKM) protein (Myc-DYKDDDDK Tag)PFKM Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 5mM DTT, 0.2 M NaCl, and 20% glycerol
- Storage
- 4 °C
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- Target
- PFKM (phosphofructokinase, Muscle (PFKM))
- Alternative Name
- PFKM (PFKM Products)
- Background
- PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: GSD7, PFK-1, PFK1, PFKA, PFKX, 6-Phosphofructokinase, muscle type 6 Phosphofructokinase Muscle Type, EC 2.7.1.11, MGC8699, PFKL, PFKM, PFKP, Phosphofructo 1 Kinase Isozyme A, Phosphofructokinase 1, Phosphofructokinase M, Phosphofructokinase, muscle, Phosphofructokinase, muscle type, Phosphofructokinase, polypeptide X, Phosphohexokinase. NCBI no.: NP_000280
- Molecular Weight
- 87.3 kDa (800aa), confirmed by MALDI-TOF
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
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