TRPT1 Protein (AA 1-253) (His tag)
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- Target See all TRPT1 Proteins
- TRPT1 (tRNA Phosphotransferase 1 (TRPT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-253
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TRPT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- TRPT1, 1-253aa, Human, His tag, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product TRPT1 Protein
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tRNA Phosphotransferase 1 (TRPT1) (AA 1-253) protein (His tag)
TRPT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
tRNA Phosphotransferase 1 (TRPT1) (AA 1-204) protein (GST tag)TRPT1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
tRNA Phosphotransferase 1 (TRPT1) (AA 1-253) protein (Strep Tag)Crystallography grade TRPT1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
tRNA Phosphotransferase 1 (TRPT1) (AA 1-249) protein (Strep Tag)Crystallography grade TRPT1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
tRNA Phosphotransferase 1 (TRPT1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)TRPT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
- Storage
- 4 °C
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- Target
- TRPT1 (tRNA Phosphotransferase 1 (TRPT1))
- Alternative Name
- TRPT1 (TRPT1 Products)
- Synonyms
- AU079016 Protein, AW045591 Protein, Tpt1 Protein, Tpt1h Protein, zgc:113138 Protein, tRNA phosphotransferase 1 Protein, TRPT1 Protein, trpt1 Protein, Trpt1 Protein
- Background
- TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: tRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1. NCBI no.: NP_001153862
- Molecular Weight
- 30.3 kDa (277aa), confirmed by MALDI-TOF
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