ATG4B Protein (AA 1-393) (His tag)
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- Target See all ATG4B Proteins
- ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-393
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ATG4B protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- ATG4B, 1-393aa, Human, His tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product ATG4B Protein
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Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-393) protein (His tag)
ATG4B Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-393) protein (GST tag)ATG4B Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-393) protein (Strep Tag)Crystallography grade ATG4B Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Autophagy related 4B Cysteine Peptidase (ATG4B) (AA 1-393) protein (Strep Tag)Crystallography grade ATG4B Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Autophagy related 4B Cysteine Peptidase (ATG4B) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ATG4B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Autophagy related 4B Cysteine Peptidase (ATG4B) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ATG4B Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF
- Storage
- 4 °C
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- Target
- ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))
- Alternative Name
- ATG4B (ATG4B Products)
- Synonyms
- apg4b Protein, APG4B Protein, AUTL1 Protein, 2510009N07Rik Protein, AW048066 Protein, Apg4b Protein, Atg4bl Protein, Autl1 Protein, Aut2b2 Protein, autophagy related 4B cysteine peptidase Protein, autophagy-related 4b Protein, Peptidase family C54 protein Protein, cysteine protease ATG4B Protein, ATG4 autophagy related 4 homolog B (S. cerevisiae) Protein, autophagy related 4B, cysteine peptidase Protein, autophagy related 4B, cysteine peptidase L homeolog Protein, ATG4B Protein, Atg4b Protein, AT3G59950 Protein, Tsp_08582 Protein, Tsp_11599 Protein, atg4b Protein, atg4b.L Protein
- Background
- ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: AUTL1, Autophagin-1. NCBI no.: AAH00719
- Molecular Weight
- 45.4 kDa (401aa) confirmed by MALDI-TOF
- Pathways
- Autophagy
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