TUBB Protein (GST tag)

Catalog No. ABIN6700746

Supplier Product No.: 009-001-u24s

Recombinant TUBB protein expressed in Escherichia coli (E. coli).

Quick Overview for TUBB Protein (GST tag) (ABIN6700746)

Target: TUBB (Tubulin, beta (TUBB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >85%

Product Details

Purification tag / Conjugate: This TUBB protein is labelled with GST tag.

Supplier Product No.: 009-001-u24s

Supplier: Rockland

Purpose: Tubulin beta (TUBB1) recombinant protein-GST fusion protein

Purification: Recombinant full-length human TUBB1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Application Details

Application Notes:

Application Note: TUBB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. TUBB1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~76 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: TUBB1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: TUBB

Background:

Synonyms: M40, TUBB, TUBB5, MGC16435, MGC117247, OK/SW-cl.56, Tubulin beta chain, Tubulin beta-5 chain

Background: TUBB1 or tubulin-beta 1 protein is a major constituent of microtubules. TUBB1 interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function (1). Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both alpha and beta tubulin monomers. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance (2). TUBB1 Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cardiovascular Disease, and Neurobiology research.

NCBI Accession: NM_178014

Pathways: Microtubule Dynamics, M Phase