MECP2 Protein (AA 1-486) (GST tag)
(1 reference)-
- Target See all MECP2 Proteins
- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-486
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This MECP2 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- MECP2 (Human) Recombinant Protein (P01)
- Sequence
- MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK APGTGRGRGR PKGSGTTRPK AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT TSTQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS PPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT SPPEPQDLSS SVCKEEKMPR GGSLESDGCP KEPAKTQPAV ATAATAAEKY KHRGEGERKD IVSSSMPRPN REEPVDSRTP VTERVS
- Characteristics
- Human MECP2 full-length ORF ( NP_004983.1, 1 a.a. - 486 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Methyl CpG Binding Protein 2 (MECP2) (AA 329-486) protein (His tag)
MECP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) protein (GST tag)MECP2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Methyl CpG Binding Protein 2 (MECP2) (AA 1-486) protein (Strep Tag)Crystallography grade MECP2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Methyl CpG Binding Protein 2 (MECP2) (AA 1-484) protein (Strep Tag)Crystallography grade MECP2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior." in: Nature neuroscience, Vol. 19, Issue 11, pp. 1497-1505, (2016) (PubMed).
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: "Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior." in: Nature neuroscience, Vol. 19, Issue 11, pp. 1497-1505, (2016) (PubMed).
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- Target
- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
- Alternative Name
- MECP2 (MECP2 Products)
- Synonyms
- AUTSX3 Protein, MRX16 Protein, MRX79 Protein, MRXS13 Protein, MRXSL Protein, PPMX Protein, RS Protein, RTS Protein, RTT Protein, 1500041B07Rik Protein, BB130002 Protein, D630021H01Rik Protein, Mbd5 Protein, WBP10 Protein, wu:fk96a04 Protein, zgc:111857 Protein, methyl-CpG binding protein 2 Protein, methyl CpG binding protein 2 Protein, methyl-CpG binding protein 2 S homeolog Protein, MECP2 Protein, Mecp2 Protein, mecp2 Protein, mecp2.S Protein
- Background
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Full Gene Name: methyl CpG binding protein 2 (Rett syndrome)
Synonyms: AUTSX3,DKFZp686A24160,MRX16,MRX79,MRXS13,MRXSL,PPMX,RTS,RTT - Gene ID
- 4204
- NCBI Accession
- NM_004992
- Pathways
- Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
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