SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)

Catalog No. ABIN6952454

Product Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.

Sequence: AA 1-419

Characteristics: 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C5227) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # YP_009724397.2). This protein carries a polyhistidine tag at the C-terminus.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.

References

Sasisekharan, Pentakota, Jayaraman, Tharakaraman, Wogan, Narayanasami: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).

Supekar, Shajahan, Gleinich, Rouhani, Heiss, Chapla, Moremen, Azadi: "Variable post-translational modifications of SARS-CoV-2 nucleocapsid protein." in: Glycobiology, (2021) (PubMed).

Qian, Lei, Patel, Lee, Monaghan-Nichols, Xin, Qiu, Fu: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Rudberg, Havervall, Månberg, Jernbom Falk, Aguilera, Ng, Gabrielsson, Salomonsson, Hanke, Murrell, McInerney, Olofsson, Andersson, Hellström, Bayati, Bergström, Pin, Sjöberg, Tegel, Hedhammar et al.: "SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5064, (2020) (PubMed).

Target Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Products)

Target Type: Viral Protein

Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Molecular Weight: 47.3 kDa