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SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin)

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
Catalog No. ABIN6952456
  • Target See all SARS-CoV-2 Spike S1 Proteins
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Protein Characteristics
    RBD
    Origin
    • 94
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 86
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His tag,AVI tag,Biotin.
    Sequence
    AA 319-541
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    Biotinylated 2019-nCoV (COVID-19) S protein RBD, His,Avitag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1). Predicted N-terminus: Arg 319 This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
    Purity
    > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike S1 Protein
  • Application Notes
    The protein has a calculated MW of 28.7 kDa. The protein migrates as 34-37 kDa under reducing (R) condition due to glycosylation.
    Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
  • Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

    Bell, Powell, Rodriguez, Cochran, Kim: "Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library." in: Protein science : a publication of the Protein Society, Vol. 30, Issue 4, pp. 716-727, (2021) (PubMed).

    Prieto-Fernández, Egia-Mendikute, Vila-Vecilla, Bosch, Barreira-Manrique, Lee, García-Del Río, Antoñana-Vildosola, Jiménez-Lasheras, Moreno-Cugnon, Jiménez-Barbero, Berra, Ereño-Orbea, Palazon: "Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate." in: Emerging microbes & infections, Vol. 10, Issue 1, pp. 1065-1076, (2021) (PubMed).

    Mulligan, Lyke, Kitchin, Absalon, Gurtman, Lockhart, Neuzil, Raabe, Bailey, Swanson, Li, Koury, Kalina, Cooper, Fontes-Garfias, Shi, Türeci, Tompkins, Walsh, Frenck, Falsey, Dormitzer, Gruber, Şahin et al.: "Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults. ..." in: Nature, Vol. 586, Issue 7830, pp. 589-593, (2020) (PubMed).

    Guo, Yao, Yang, Zhang, Qi, Gou, Xi, Liu, Zhang, Cheng, Wang, Rong, Chen, Kong: "Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection." in: Chemical engineering journal (Lausanne, Switzerland : 1996), pp. 127742, (2020) (PubMed).

  • Target
    SARS-CoV-2 Spike S1
    Abstract
    SARS-CoV-2 Spike S1 Products
    Synonyms
    E2 Protein, Surface Glycoprotein Protein, S Protein
    Target Type
    Viral Protein
    Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    28.2 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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