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SARS-CoV-2 Spike S1 Protein (His tag,AVI tag,Biotin)

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
Catalog No. ABIN6952457
  • Target See all SARS-CoV-2 Spike S1 Proteins
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Origin
    • 94
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 86
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His tag,AVI tag,Biotin.
    Sequence
    AA 16-685
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    Biotinylated 2019-nCoV (COVID-19) S1 protein, His,Avitag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). Predicted N-terminus: Val 16
    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
    Purity
    > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike S1 Protein
  • Application Notes
    The protein has a calculated MW of 78.6 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
    Comment

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
  • Kaneko, Satta, Komuro, Muthukrishnan, Kakarla, Guo, An, Elahi, Kornblum, Liebeskind, Hsiai, Hinman: "Flow-Mediated Susceptibility and Molecular Response of Cerebral Endothelia to SARS-CoV-2 Infection." in: Stroke, Vol. 52, Issue 1, pp. 260-270, (2021) (PubMed).

    Prieto-Fernández, Egia-Mendikute, Vila-Vecilla, Bosch, Barreira-Manrique, Lee, García-Del Río, Antoñana-Vildosola, Jiménez-Lasheras, Moreno-Cugnon, Jiménez-Barbero, Berra, Ereño-Orbea, Palazon: "Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate." in: Emerging microbes & infections, Vol. 10, Issue 1, pp. 1065-1076, (2021) (PubMed).

    Ng, Attig, Bolland, Young, Major, Wrobel, Gamblin, Wack, Kassiotis: "Tissue-specific and interferon-inducible expression of nonfunctional ACE2 through endogenous retroelement co-option." in: Nature genetics, Vol. 52, Issue 12, pp. 1294-1302, (2020) (PubMed).

  • Target
    SARS-CoV-2 Spike S1
    Abstract
    SARS-CoV-2 Spike S1 Products
    Synonyms
    E2 Protein, Surface Glycoprotein Protein, S Protein
    Target Type
    Viral Protein
    Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    78.6 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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