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SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)

SARS-CoV-2 N Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Catalog No. ABIN6952460
  • Target See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) Proteins
    SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
    Protein Type
    Recombinant
    Origin
    • 54
    • 6
    • 5
    • 1
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 29
    • 23
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.
    Sequence
    AA 1-419
    Characteristics
    2019-nCoV (COVID-19) Nucleocapsid protein, His Tag (NUN-C5227) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # YP_009724397.2). This protein carries a polyhistidine tag at the C-terminus.
    Purity
    >95 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 N Protein
  • Application Notes
    The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-65 kDa under reducing (R) condition due to glycosylation.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    -20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
  • Sasisekharan, Pentakota, Jayaraman, Tharakaraman, Wogan, Narayanasami: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).

    Supekar, Shajahan, Gleinich, Rouhani, Heiss, Chapla, Moremen, Azadi: "Variable post-translational modifications of SARS-CoV-2 nucleocapsid protein." in: Glycobiology, (2021) (PubMed).

    Qian, Lei, Patel, Lee, Monaghan-Nichols, Xin, Qiu, Fu: "Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin." in: bioRxiv : the preprint server for biology, (2021) (PubMed).

    Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

    Rudberg, Havervall, Månberg, Jernbom Falk, Aguilera, Ng, Gabrielsson, Salomonsson, Hanke, Murrell, McInerney, Olofsson, Andersson, Hellström, Bayati, Bergström, Pin, Sjöberg, Tegel, Hedhammar et al.: "SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5064, (2020) (PubMed).

  • Target
    SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
    Alternative Name
    SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Products)
    Synonyms
    nucleocapsid phosphoprotein Protein, N Protein
    Target Type
    Viral Protein
    Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
    Molecular Weight
    47.3 kDa
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