ACE2 Protein (Fc Tag)

Catalog No. ABIN6952465

Product Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ACE2 protein is labelled with Fc Tag.

Sequence: AA 18-740

Characteristics: Human ACE2, Fc Tag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1). Predicted N-terminus: Gln 18
This protein carries a human IgG1 Fc tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: The protein has a calculated MW of 110.0 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, Arginine, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.

References

Huang, Zhou, He, Chiem, Mabrouk, Nissly, Bird, Strauss, Sambhara, Ortega, Wohlfert, Martinez-Sobrido, Kuchipudi, Davidson, Lovell: "SARS-CoV-2 RBD Neutralizing Antibody Induction is Enhanced by Particulate Vaccination." in: Advanced materials (Deerfield Beach, Fla.), Vol. 32, Issue 50, pp. e2005637, (2021) (PubMed).

Ozono, Zhang, Ode, Sano, Tan, Imai, Miyoshi, Kishigami, Ueno, Iwatani, Suzuki, Tokunaga: "SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity." in: Nature communications, Vol. 12, Issue 1, pp. 848, (2021) (PubMed).

Wei, Duan, Liu, Wang, Ding, Chen, Xie, Tian, Yu, Ge, Zhang, Chen, Li, Meng: "Chicken Egg Yolk Antibodies (IgYs) block the binding of multiple SARS-CoV-2 spike protein variants to human ACE2." in: International immunopharmacology, Vol. 90, pp. 107172, (2021) (PubMed).

Leach, Ilca, Akbar, Ferrari, Bentley, Mattiuzzo, Onuoha, Miller, Ali, Rabbitts: "A tetrameric ACE2 protein broadly neutralizes SARS-CoV-2 spike variants of concern with elevated potency." in: Antiviral research, Vol. 194, pp. 105147, (2021) (PubMed).

Guo, Yao, Yang, Zhang, Qi, Gou, Xi, Liu, Zhang, Cheng, Wang, Rong, Chen, Kong: "Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection." in: Chemical engineering journal (Lausanne, Switzerland : 1996), pp. 127742, (2020) (PubMed).

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2 (ACE2 Products)

Synonyms: ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein

Background: Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Molecular Weight: 110.0 kDa

NCBI Accession: NP_068576

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour