SARS-CoV-2 Spike (Trimer) (Active) protein (His tag)

Details for Product No. ABIN6952523
Protein Name
  • E2
  • Surface Glycoprotein
  • S
Protein Characteristics
Trimer
4
3
2
1
Origin
SARS Coronavirus-2 (SARS-CoV-2)
17
Source
HEK-293 Cells
12
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This SARS-CoV-2 Spike protein is labelled with His tag.
Options
Purpose 2019-nCoV (COVID-19) Full Length S protein (R683A, R685A), His Tag, active trimer (MALS verified)
Specificity
  • Purity of 2019-nCoV (COVID-19) full length S protein is higher than 85%, and the molecular weight is 640-680 kDa as verified by SEC-MALS.
  • The affinity constant of human ACE2 protein binding to 2019-nCoV (COVID-19) S protein full length trimer protein was 35.3 nM as verified by SPR.
  • The linear range of biotinylated human ACE2 protein binding to 2019-nCoV (COVID-19) S protein full length trimer protein was 4-63 ng/mL as verified by ELISA.
Characteristics 2019-nCoV Full Length S protein (R683A, R685A), His Tag, active trimer is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A, R685A)). Predicted N-terminus: Val 16
This protein carries a polyhistidine tag at the C-terminus.

The two site mutations (R683A, R685A) in the SARS-CoV-2 Spike protein are changing the protease cleavage site, so that expression of the full length Spike protein is possible.
The two site mutations should not affect the binding between Spike protein RBD and ACE2 or the binding between Spike protein RBD and its specifc antibodies.
Purity > 90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Protein Name
Background Protein S (PROS1) is lycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Gene ID 43740568
UniProt P0DTC2
Application Notes The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-230 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Comment

An article published in Science determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation. To facilitate medical countermeasure development, we have developed the full length S protein with a trimer structure. The molecular weight and polymer form of the protein was verified by SEC-MALS technology. We also offer high quality 2019-nCoV S1, S RBD, N, human ACE2 and other proteins. The bioactivity was verified by SPR and BLI.

Restrictions For Research Use only
Format Liquid
Buffer Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.
Handling Advice Please avoid repeated freeze-thaw cycles.
Storage -20 °C,-80 °C
Storage Comment For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:-20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
Expiry Date 12 months
Supplier Images
Image no. 1 for SARS-CoV-2 Spike (Trimer) (Active) protein (His tag) (ABIN6952523) Human ACE2, Fc Tag (MALS verified) (ABIN6952459) Captured on CM5 chip via anti-human ...
Image no. 2 for SARS-CoV-2 Spike (Trimer) (Active) protein (His tag) (ABIN6952523) 2019-nCoV Full Length S protein (R683A, R685A), His Tag, active trimer on SDS-PAGE un...
Image no. 3 for SARS-CoV-2 Spike (Trimer) (Active) protein (His tag) (ABIN6952523) The purity of 2019-nCoV Full Length S protein (R683A, R685A), His Tag, active trimer ...
Image no. 4 for SARS-CoV-2 Spike (Trimer) (Active) protein (His tag) (ABIN6952523) Immobilized Biotinylated Human ACE2, Fc,Avitag (ABIN6952458) at 1 μg/mL (100 μL/well)...
Image no. 5 for SARS-CoV-2 Spike (Trimer) (Active) protein (His tag) (ABIN6952523) Immobilized 2019-nCoV Full Length S protein (R683A, R685A), His Tag, active trimer (A...
Did you look for something else?