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SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag)

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. SDS, ELISA
Catalog No. ABIN6952630
  • Target See all SARS-CoV-2 Spike S1 Proteins
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Protein Characteristics
    RBD, V367F
    Origin
    • 94
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 86
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), ELISA
    Purpose
    SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag
    Sequence
    AA 319-541
    Characteristics
    SARS-CoV-2 S protein RBD (V367F), His Tag (SPD-S52H4) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (V367F). Predicted N-terminus: Arg 319
    This protein carries a polyhistidine tag at the C-terminus.
    The V367F mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.
    Purity
    >95 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike S1 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    This product is stable after storage at:
    4-8°C for 12 months in lyophilized state,
    -70°C for 3 years under sterile conditions after reconstitution.
  • Guo, Huang, Zhang, Yao, Zhou, Shen, Shen, Li, Li, Zhang, Chen, Chen, Wu, Fu, Zeng, Feng, Pi, Wang, Zhou, Lu, Li, Fang, Lu, Hu, Wang, Zhang, Gao, Adrian, Wang, Yu, Peng, Gabibov, Min, Wang, Huang et al.: "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. ..." in: Nature communications, Vol. 12, Issue 1, pp. 2623, (2021) (PubMed).

  • Target
    SARS-CoV-2 Spike S1
    Abstract
    SARS-CoV-2 Spike S1 Products
    Synonyms
    E2 Protein, Surface Glycoprotein Protein, S Protein
    Target Type
    Viral Protein
    Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    27.0 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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