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SARS-CoV-2 Spike S1 (N354D), (RBD) protein (His tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. ELISA, SDS
Pubmed (1)
Catalog No. ABIN6952631
$490.00
Plus shipping costs $45.00 and $24.00 dry ice
100 μg ABIN6952631
100 μg ABIN6952631
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  • Target
    SARS-CoV-2 Spike S1
    Protein Type
    Recombinant
    Protein Characteristics
    N354D, RBD
    Origin
    • 70
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 61
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike S1 protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS)
    Purpose
    SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag
    Sequence
    AA 319-541
    Characteristics
    SARS-CoV-2 S protein RBD (N354D), His Tag (SPD-S52H5) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (N354D)). Predicted N-terminus: Arg 319
    This protein carries a polyhistidine tag at the C-terminus.
    Purity
    >95 % as determined by SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    This product is stable after storage at:
    4-8°C for 12 months in lyophilized state,
    -70°C for 3 years under sterile conditions after reconstitution.
  • Yan Lou, Wenxiang Zhao, Haitao Wei, Min Chu, Ruihua Chao, Hangping Yao, Junwei Su, Yanan Li, Xiulan Li, Yu Cao, Yanyan Feng, Ping Wang, Yongyang Xia, Yushuan Shang, Fengping Li, Pingju Ge, Xinglin Zhang, Wenjing Gao, Bing Du, Tingbo Liang, Yunqing Qiu, Mi: "Cross-neutralization antibodies against SARS-CoV-2 and RBD mutations from convalescent patient antibody libraries" in: bioRxiv, 2020

  • Target
    SARS-CoV-2 Spike S1
    Synonyms
    E2, Surface Glycoprotein, S
    Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    27.0 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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