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SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag) Protein

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN6952670
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  • Target See all SARS-CoV-2 Spike Proteins
    SARS-CoV-2 Spike
    Protein Type
    Recombinant
    Protein Characteristics
    Trimer
    Origin
    • 349
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    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 309
    • 31
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    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Purpose
    Trimeric, full length Cov-2 spike protein for assay development (""Antibody tests"")
    Specificity
    • single span transmembrane membrane protein (aa 1-1273)
    • Furin cleavage site ""RRAR"" mutated to ""GSAS""
    • trimerization (3 x 142 kDa) shown on native PAGE
    • expressed in Expi293™ cells
    • C-terminal Rho1D4 tag for affinity purification
    • Solubilization and stabilization in LMNG detergent
    • 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Full length SARS Cov-2 spike protein expressed in Expi293™ cells to assure native state glycosylation.
    • Purification by a multi-step, protein-specific protocol to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE.
    Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Protein is endotoxin-free.
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Further modifications:
    - furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
    - C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
    Size: 1286 amino acids (including Rho1D4 tag and linker)

    Restrictions
    For Research Use only
  • Buffer
    20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SARS-CoV-2 Spike
    Abstract
    SARS-CoV-2 Spike Products
    Synonyms
    E2, E2 glycoprotein precursor, Surface Glycoprotein, S
    Target Type
    Viral Protein
    Background
    The spike glycoprotein exists as a homotrimeric fusion protein. Each of the trimers contains 66 glycosylation sites for host-derived N-linked glycans. Accordingly, expression of this primary target for SARS-CoV-2 vaccine development in an appropriate, human expression system is of utmost importance. Prior to ACE2 binding, each monomer in the prefusion complex contains an S1 ectodomain including the receptor binding domain (RBD) and an S2 endodomain harboring a transmembrane domain. In the predominant state of the trimer, one of the RBDs is in an “up” position whereas the other two are in a “down” position. Interaction of S-protein and ACE2 only takes place with the RBD in the “up” position. Receptor binding triggers a structural change that leads to separation of the S1 and S2 subunits.
    Molecular Weight
    3 x 142 kDa
    Gene ID
    43740568
    UniProt
    P0DTC2
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