SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) protein (His tag)

Catalog No. ABIN6952706

Product Details

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Purpose: SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag

Sequence: AA 1-198

Characteristics: SARS-CoV-2 NSP8, His Tag (NS8-C5149) is expressed from E.coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1).Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Tris, 250 mM NaCl, pH 8.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.

Target Details

Target: SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)

Alternative Name: NSP8 (SARS-CoV NSP8 Products)

Synonyms: pp1a Protein, pp1ab Protein, orf1ab polyprotein (pp1ab) Protein, orf1ab Protein

Target Type: Viral Protein

Background: NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of SARS-CoV-2, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.

Molecular Weight: 23.9 kDa

NCBI Accession: YP_009725304