SARS-CoV-2 NSP16, NSP10 protein (His tag,Twin-Strep tag)
-
- Target
- SARS-CoV-2 NSP16, NSP10
- Protein Type
- Recombinant
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag,Twin-Strep tag
- Purpose
- SARS-CoV-2 (COVID-19) NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag
- Sequence
- AA 1-298
- Characteristics
-
SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag (NS0-C51W3) is expressed from E.coli cells. It contains AA Ser 1 - Asn 298 (NSP16) & Ala 1 - Gln 139 (NSP10) (Accession # YP_009725311.1 (NSP16) & YP_009725306.1 (NSP10). Predicted N-terminus: Met (NSP16) & Met (NSP10)
SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag is produced by co-expression of NSP16 and NSP10, has a calculated MW of 35.2 kDa (NSP16) and 17.4 kDa (NSP10). Subunit NSP16 is fused with a polyhistidine tag at the N-terminus and subunit NSP10 is fused with a Twin Strep tag at the N-terminus. - Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- -80 °C
- Storage Comment
-
The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.
-
- Target
- SARS-CoV-2 NSP16, NSP10
- Alternative Name
- SARS-CoV-2 NSP16 & NSP10
- Target Type
- Viral Protein
- Background
- NSP10, Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and 2'-O-methyltransferase (NSP16) activities. Therefore plays an essential role in viral mRNAs cap methylation. 2'-O-methyltransferase (NSP16) that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16, these interactions enhance nsp14 and nsp16 enzymatic activities.
- Molecular Weight
- 35.3 kDa (NSP16) & 18.3 kDa (NSP10)
-