SARS-CoV-2 Spike Protein (B.1.1.7 - alpha) (rho-1D4 tag)

Catalog No. ABIN6963743

Product Details

Target: SARS-CoV-2 Spike

Protein Characteristics: B.1.1.7 - alpha

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.

Application: ELISA, Ligand Binding Assay (LBA)

Purpose: This is the spike protein of the mutant strain B.1.1.7, also commonly known as the "UK / Great Britain mutant".

Sequence: Full-length sequence (aa 1 - 1273), del 69, del 70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
furin cleavage site ""RRAR"" mutated to ""GSAG"", KV986PP
C-terminal Rho1D4 tag fused with spacer ""GSSG"" to protein sequence

Specificity: Mutation that differ from canonical sequence of SPIKE protein: del 69-70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H

Characteristics: "SARS CoV-2 full-length Spike B.1.1.7 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent.

Purification: affinity chromatography

Purity: > 98 % as determined by SDS-PAGE

Application Details

Application Notes: ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses

Comment:

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG

Storage: -80 °C

Storage Comment: Store at -80°C. Avoid freeze-thaw cycles

References

Bhalla, Payam, Morelli, Sharma, Johnson, Thomson, Jolly, Canfarotta: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).

Funari, Bhalla, Gentile: "Measuring the Radius of Gyration and Intrinsic Flexibility of Viral Proteins in Buffer Solution Using Small-Angle X-ray Scattering." in: ACS measurement science au, Vol. 2, Issue 6, pp. 547-552, (2022) (PubMed).

Target Details

Target: SARS-CoV-2 Spike

Alternative Name: SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Molecular Weight: 142114 Da

UniProt: P0DTC2