SARS-CoV-2 Spike Protein (B.1.525 - eta) (rho-1D4 tag)

Catalog No. ABIN6972924

Product Details

Key Features:

• Recombinant trimeric S protein of the SARS-CoV-2 variant of interest B.1.525.
• Contains Q52R, del 69-70, del 144, E484K, F888L, and Q677H mutations characteristic for the SARS-CoV-2 eta variant (B.1.525).

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: B.1.525 - eta

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Eta

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.

Application: ELISA, Ligand Binding Assay (LBA)

Purpose: SARS CoV-2 full-length Spike B.1.525 Mutation

Sequence: MFVFLVLLPL VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TRDLFLPFFS NVTWFHAISG TNGTKRFDNP VLPFNDGVYF ASTEKSNIIR GWIFGTTLDS KTQSLLIVNN ATNVVIKVCE FQFCNDPFLG VYHKNNKSWM ESEFRVYSSA NNCTFEYVSQ PFLMDLEGKQ GNFKNLREFV FKNIDGYFKI YSKHTPINLV RDLPQGFSAL EPLVDLPIGI NITRFQTLLA LHRSYLTPGD SSSGWTAGAA AYYVGYLQPR TFLLKYNENG TITDAVDCAL DPLSETKCTL KSFTVEKGIY QTSNFRVQPT ESIVRFPNIT NLCPFGEVFN ATRFASVYAW NRKRISNCVA DYSVLYNSAS FSTFKCYGVS PTKLNDLCFT NVYADSFVIR GDEVRQIAPG QTGKIADYNY KLPDDFTGCV IAWNSNNLDS KVGGNYNYLY RLFRKSNLKP FERDISTEIY QAGSTPCNGV KGFNCYFPLQ SYGFQPTNGV GYQPYRVVVL SFELLHAPAT VCGPKKSTNL VKNKCVNFNF NGLTGTGVLT ESNKKFLPFQ QFGRDIADTT DAVRDPQTLE ILDITPCSFG GVSVITPGTN TSNQVAVLYQ DVNCTEVPVA IHADQLTPTW RVYSTGSNVF QTRAGCLIGA EHVNNSYECD IPIGAGICAS YQTHTNSPGS ASSVASQSII AYTMSLGAEN SVAYSNNSIA IPTNFTISVT TEILPVSMTK TSVDCTMYIC GDSTECSNLL LQYGSFCTQL NRALTGIAVE QDKNTQEVFA QVKQIYKTPP IKDFGGFNFS QILPDPSKPS KRSFIEDLLF NKVTLADAGF IKQYGDCLGD IAARDLICAQ KFNGLTVLPP LLTDEMIAQY TSALLAGTIT SGWTLGAGAA LQIPFAMQMA YRFNGIGVTQ NVLYENQKLI ANQFNSAIGK IQDSLSSTAS ALGKLQDVVN QNAQALNTLV KQLSSNFGAI SSVLNDILSR LDPPEAEVQI DRLITGRLQS LQTYVTQQLI RAAEIRASAN LAATKMSECV LGQSKRVDFC GKGYHLMSFP QSAPHGVVFL HVTYVPAQEK NFTTAPAICH DGKAHFPREG VFVSNGTHWF VTQRNFYEPQ IITTDNTFVS GNCDVVIGIV NNTVYDPLQP ELDSFKEELD KYFKNHTSPD VDLGDISGIN ASVVNIQKEI DRLNEVAKNL NESLIDLQEL GKYEQYIKWP WYIWLGFIAG LIAIVMVTIM LCCMTSCCSC LKGCCSCGSC CKFDEDDSEP VLKGVKLHYT GSSGTETSQV APA

Specificity: Mutation that differ from "original" SPIKE protein: Q52R, del 69-70, del 144, E484K, F888L, Q677H

Characteristics: "SARS CoV-2 full-length Spike B.1.525 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer these mutant SPIKE proteins.

Purification: Affinity Chromatography

Purity: >98% as determined by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG

Storage: -80 °C

Storage Comment: Store at -80°C. Avoid freeze-thaw cycles

Target Details

Target: SARS-CoV-2 Spike

Alternative Name: SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Molecular Weight: 141683.90 Da

UniProt: P0DTC2