GUCY2C Protein (AA 24-430) (Fc Tag,AVI tag,Biotin)

Catalog No. ABIN6973083

Product Details

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-430

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This GUCY2C protein is labelled with Fc Tag,AVI tag,Biotin.

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Biotinylated Human GUCY2C / Guanylyl cyclase C Protein, Fc,Avitag™

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Target Details

Target: GUCY2C (Guanylate Cyclase 2C (Heat Stable Enterotoxin Receptor) (GUCY2C))

Alternative Name: GUCY2C (GUCY2C Products)

Synonyms: DIAR6 Protein, GUC2C Protein, MECIL Protein, MUCIL Protein, STAR Protein, AI893437 Protein, GC-C Protein, Gcc Protein, GCC Protein, gucy2c-A Protein, GUCY2C Protein, Gucy2c Protein, guanylate cyclase 2C Protein, guanylate cyclase 2c Protein, guanylate cyclase 2C L homeolog Protein, GUCY2C Protein, Gucy2c Protein, gucy2c.L Protein, gucy2c Protein

Background: GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

Molecular Weight: 74.1 kDa

NCBI Accession: NP_004954