SARS-CoV-2 Spike Protein (B.1.617.1 - kappa) (rho-1D4 tag)

Catalog No. ABIN6976302

Product Details

Key Features:

• Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.617.
• Contains E154K, L452R, E484Q, D614G, and P681R mutations characteristic for the SARS-CoV-2 kappa variant (B.1.617.1).

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: B.1.617.1 - kappa

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.

Application: ELISA, Ligand Binding Assay (LBA)

Purpose: This is the spike protein of the mutant strain B.1.617, also commonly known as the "India" or "Kappa mutant".

Sequence: 1286 amino acids (including Rho1D4 tag and linker)

Specificity: Mutation that differ from "original" SPIKE protein: G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H

Characteristics: "SARS CoV-2 full-length Spike B.1.617 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer these mutant SPIKE proteins.
We offer the SPIKE protein of the mutants in full-length and active in their native trimeric form, stabilized with the LMNG detergent.

Purity: > 98% as determined by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG

Storage: -80 °C

Storage Comment: Store at -80°C. Avoid freeze-thaw cycles

Target Details

Target: SARS-CoV-2 Spike

Alternative Name: SARS2 Spike glycoprotein (SARS-CoV-2 Spike Products)

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Background: All viruses undergo fast mutations and adapt quickly to the countermeasures that the immune systems creates. SARS-CoV-2 of the COVID-19 pandemic is no exception to this. During the pandemic multiple mutant strains arose.

Molecular Weight: 142190.43 Da

UniProt: P0DTC2