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SARS-CoV-2 Spike Protein (B.1.429 - epsilon, RBD) (His tag,AVI tag,Biotin)

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Epsilon Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Catalog No. ABIN7013308
  • Target See all SARS-CoV-2 Spike Proteins
    SARS-CoV-2 Spike
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    B.1.429 - epsilon, RBD
    Origin
    • 223
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    SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Epsilon
    Source
    • 198
    • 7
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    HEK-293 Cells
    Purification tag / Conjugate
    This SARS-CoV-2 Spike protein is labelled with His tag,AVI tag,Biotin.
    Purpose
    Biotinylated SARS-CoV-2 Spike RBD (L452R), His,Avitag™ (MALS verified)
    Sequence
    AA 319-537
    Specificity
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Characteristics
    Biotinylated SARS-CoV-2 Spike RBD (L452R), His,Avitag is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R). The L452R mutation was identified in the SARS-CoV-2 variant (known as B.1.427/B.1.429) which emerged in California, the U.S.
    Purity
    >90 % as determined by SDS-PAGE.
    Endotoxin Level
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike Protein
  • Comment

    Ready-to-use Avitag™ biotinylated protein:
    The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Storage
    -20 °C
  • Target
    SARS-CoV-2 Spike
    Alternative Name
    SARS-CoV-2 S protein (SARS-CoV-2 Spike Products)
    Synonyms
    E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
    Target Type
    Viral Protein
    Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    Molecular Weight
    28.3 kDa
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