ITCH Protein (AA 197-542) (His-IF2DI Tag)
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- Target See all ITCH Proteins
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 197-542
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ITCH protein is labelled with His-IF2DI Tag.
- Application
- Western Blotting (WB), ELISA
- Purity
- Greater than 95 % by SDS-PAGE gel analyses
- Sterility
- 0.2 μm filtered
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Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) protein (GST tag)
ITCH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 2-903) protein (His tag)Crystallography grade ITCH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 2-864) protein (His tag)Crystallography grade ITCH Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex
- Buffer
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % trehalose, pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution
- Expiry Date
- 12 months
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- Target
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- Alternative Name
- ITCH (ITCH Products)
- Synonyms
- AIF4 Protein, AIP4 Protein, NAPP1 Protein, dJ468O1.1 Protein, 6720481N21Rik Protein, 8030492O04Rik Protein, A130065M08 Protein, C230047C07Rik Protein, itchy E3 ubiquitin protein ligase Protein, itchy, E3 ubiquitin protein ligase Protein, ITCH Protein, Itch Protein
- Background
- AIF4, AIP4, AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)), atrophin-1 interacting protein 4, Atrophin-1-interacting protein 4, dJ468O1.1, EC 6.3.2, EC 6.3.2.-, ITCH, itchy (Mouse homolog) E3 ubiquitin protein ligase
- Molecular Weight
- 59.5 kDa
- UniProt
- Q96J02
- Pathways
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
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