DDX11 Protein (His tag)

Catalog No. ABIN7318007

Product Details

Target: DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DDX11 protein is labelled with His tag.

Purpose: Recombinant Human CHL-1 Protein (His Tag)(Active)

Sequence: Met 1-Gln 1080

Characteristics: A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CHL1 coated plates (0.8 μg/ml and 100 μl/well), approximately 40%-60% will adhere specifically after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))

Alternative Name: CHL-1 (DDX11 Products)

Synonyms: CHL1 Protein, CHLR1 Protein, KRG2 Protein, WABS Protein, 4732462I11Rik Protein, essa15a Protein, putative ATP-dependent RNA helicase DDX11-like protein 8 Protein, DEAD/H-box helicase 11 Protein, DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 Protein, LOC100448472 Protein, DDX11 Protein, Ddx11 Protein

Background:

Background: Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin typeIII domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.

Synonym: CALL,L1CAM2,LICAM2

Molecular Weight: 120 kDa

Pathways: ER-Nucleus Signaling