DDC Protein (His tag)

Catalog No. ABIN7195446

Product Details

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This DDC protein is labelled with His tag.

Purpose: Recombinant Human DOPA Decarboxylase/DDC Protein (His Tag)(Active)

Sequence: Met 1-Glu 480

Characteristics: A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Alternative Name: DOPA Decarboxylase/DDC (DDC Products)

Synonyms: AADC Protein, wu:fa56d05 Protein, wu:fd59h03 Protein, wu:fk20h01 Protein, zgc:65801 Protein, zgc:76929 Protein, Ddc Protein, DdcDv Protein, Dvir\\GJ17995 Protein, GJ17995 Protein, dvir_GLEANR_2561 Protein, Aadc Protein, 5-HT Protein, CG10697 Protein, DDC Protein, DdcDm Protein, Dmel\\CG10697 Protein, ddc Protein, fDDC Protein, l(2)37Bl Protein, l(2)37Ch Protein, l(2)k02104 Protein, dopa decarboxylase Protein, Dopa-decarboxylase Protein, dopa decarboxylase (aromatic L-amino acid decarboxylase) Protein, Dopa decarboxylase Protein, dopa decarboxylase L homeolog Protein, DDC Protein, ddc Protein, Ddc Protein, Dvir\Ddc Protein, ddc.L Protein

Background:

Background: Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

Synonym: AADC

Molecular Weight: 55 kDa

NCBI Accession: NP_000781

Pathways: Dopaminergic Neurogenesis