FGFR2 Protein (AA 400-821) (GST tag,His tag)

Catalog No. ABIN7317082

Recombinant FGFR2 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for FGFR2 Protein (AA 400-821) (GST tag,His tag) (ABIN7317082)

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 92 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 400-821

Purification tag / Conjugate: This FGFR2 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human FGFR2/CD332 Protein (aa 400-821, His & GST Tag)(Active)

Sequence: Met 400-Thr 821

Characteristics: A DNA sequence encoding the human FGFR2 (NP_000132.3) cytoplasmic domain (Met 400-Thr 821) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1.The specific activity was determined to be 28 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic with a linear range of 15.6-250 ng/ml.3. Measured by its binding ability in a functional ELISA. Immobilized recombinant recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF basic with a linear range of 0.16-1.25 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Alternative Name: FGFR2/CD332

Background:

Background: FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: BBDS;BEK;BFR-1;CD332;CEK3;CFD1;ECT1;JWS;K-SAM;KGFR;TK14;TK25

Molecular Weight: 75.7 kDa

NCBI Accession: NP_000132

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding