FEN1 Protein
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- Target See all FEN1 Proteins
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human Flap Endonuclease 1/FEN1 Protein
- Sequence
- Met 1-Lys380
- Characteristics
- Recombinant Human Flap Endonuclease 1 is produced by our E.coli expression system and the target gene encoding Met1-Lys380 is expressed.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product FEN1 Protein
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Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-110) protein (GST tag)
FEN1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (GST tag)FEN1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (Strep Tag)Crystallography grade FEN1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-378) protein (Strep Tag)Crystallography grade FEN1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Flap Structure-Specific Endonuclease 1 (FEN1) protein (Myc-DYKDDDDK Tag)FEN1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, 1 mM DDT, 10 % Glycerol, pH 8.0.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Alternative Name
- Flap Endonuclease 1/FEN1 (FEN1 Products)
- Synonyms
- FEN-1 Protein, FEN1 Protein, AW538437 Protein, MF1 Protein, RAD2 Protein, cb879 Protein, fen-1 Protein, fen1 Protein, mf1 Protein, rad2 Protein, xFEN1a Protein, flap structure-specific endonuclease 1 Protein, flap endonuclease 1-like Protein, Flap endonuclease-1 Protein, 5'-3' exonuclease family protein Protein, flap structure specific endonuclease 1 Protein, flap structure-specific endonuclease 1 L homeolog Protein, nuclease, Rad2 family Protein, FEN1 Protein, LOC100808044 Protein, Bm1_49605 Protein, AT5G26680 Protein, Fen1 Protein, fen1 Protein, fen1.L Protein
- Background
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Background: Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Synonym: Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-Specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, FEN1, RAD2
- Molecular Weight
- 42.6 kDa
- UniProt
- P39748
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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