GFRA1 Protein (His tag)

Catalog No. ABIN7320387

Recombinant GFRA1 protein expressed in HEK-293 Cells.

Quick Overview for GFRA1 Protein (His tag) (ABIN7320387)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 98 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This GFRA1 protein is labelled with His tag.

Purpose: Recombinant Mouse GFRA1/GDNFRA Protein (His Tag)(Active)

Sequence: Met 1-Ser 425

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRα1 isoform beta (AAB86600.1) was expressed with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind with human GDNF in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GFRA1/GDNFRA

Background:

Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.

Synonym: AU042498

Molecular Weight: 46.3 kDa