PPP3CA Protein (His tag)

Catalog No. ABIN7197576

Product Details

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PPP3CA protein is labelled with His tag.

Purpose: Recombinant Human PPP3CA/CALNA Protein (His Tag)(Active)

Sequence: Ser2-Gln521

Characteristics: A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.

Purity: > 94 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Using the Octet RED System, the affinity constant (Kd) of human PPPP3CA-His bound to Human PPIA-His was 0.9 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Alternative Name: PPP3CA/CALNA (PPP3CA Products)

Synonyms: CALN Protein, CALNA Protein, CALNA1 Protein, CCN1 Protein, CNA1 Protein, PPP2B Protein, 2900074D19Rik Protein, AI841391 Protein, AW413465 Protein, CN Protein, Caln Protein, Calna Protein, CnA Protein, Calna1 Protein, calcineurin Protein, caln Protein, calna Protein, calna1 Protein, ccn1 Protein, cna1 Protein, ppp2b Protein, fj46e08 Protein, si:dkeyp-79c2.2 Protein, wu:fj46e08 Protein, ppp3ca Protein, protein phosphatase 3 catalytic subunit alpha Protein, protein phosphatase 3, catalytic subunit, alpha isoform Protein, protein phosphatase 3, catalytic subunit, alpha isozyme Protein, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) Protein, protein phosphatase 3, catalytic subunit, alpha isozyme L homeolog Protein, serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Protein, PPP3CA Protein, Ppp3ca Protein, ppp3ca Protein, ppp3ca.L Protein, LOC100205420 Protein

Background:

Background: PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.

Synonym: CALN,CALNA,CALNA1,CCN1,CNA1,PPP2B

Molecular Weight: 60.8 kDa

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling