Alkaline Phosphatase Protein (ALP) (AA 20-503) (His-DYKDDDDK Tag)
ALP
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target See all Alkaline Phosphatase (ALP) products
- Alkaline Phosphatase (ALP)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-503
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Alkaline Phosphatase protein is labelled with His-DYKDDDDK Tag.
- Purpose
- Human Alkaline phosphatase (Intestinal type) /ALPI Protein
- Sequence
- Val20-Asp503
- Characteristics
- Recombinant Human Alkaline phosphatase (Intestinal type) /ALPI Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val20-Asp503.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.96 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Alkaline Phosphatase (ALP) (AA 23-506) protein (His tag)
ALP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Alkaline Phosphatase (ALP) (AA 21-501) protein (His tag)ALP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Alkaline Phosphatase (ALP)
- Alternative Name
- Alkaline phosphatase (ALP Products)
- Synonyms
- ALP Protein, ALP1 Protein, CRASH Protein, ECK0378 Protein, JW0374 Protein, psiA Protein, 2410004D18Rik Protein, AU040643 Protein, AW060375 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, Actn2lp Protein, Alp Protein, asparaginase like 1 Protein, bacterial alkaline phosphatase Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, PDZ and LIM domain 3 Protein, ASRGL1 Protein, phoA Protein, Asrgl1 Protein, CCL27 Protein, alpl Protein, alp Protein, Pdlim3 Protein
- Background
- Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
- Molecular Weight
- 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
- UniProt
- P09923
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