AXL Protein (AA 26-449) (Fc Tag,Biotin)

Catalog No. ABIN7273883

Product Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Protein Type: Recombinant

Protein Characteristics: AA 26-449

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This AXL protein is labelled with Fc Tag,Biotin.

Purpose: Biotinylated Human Axl Protein (Primary Amine Labeling)

Sequence: Ala26-Pro449

Characteristics: Recombinant Biotinylated Human Axl Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala26-Pro449.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Axl Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Alternative Name: AXL (AXL Products)

Background: Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Molecular Weight: 72.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Cellular Response to Molecule of Bacterial Origin