CD86 Protein (CD86) (AA 26-240) (His tag)

Catalog No. ABIN7273896

Product Details

Target: CD86

Protein Type: Recombinant

Protein Characteristics: AA 26-240

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CD86 protein is labelled with His tag.

Purpose: Cynomolgus B7-2/CD86 Protein

Sequence: Tyr26-His240

Characteristics: Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr26-His240.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.102 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD86

Alternative Name: B7-2 (CD86 Products)

Synonyms: B7-2 Protein, B7.2 Protein, B70 Protein, CD28LG2 Protein, LAB72 Protein, B7 Protein, CLS1 Protein, Cd28l2 Protein, ETC-1 Protein, Ly-58 Protein, Ly58 Protein, MB7 Protein, MB7-2 Protein, TS/A-2 Protein, CD86 molecule Protein, CD86 antigen Protein, integrin subunit alpha 2b Protein, CD86 Protein, Cd86 Protein, Itga2b Protein

Background: B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Molecular Weight: 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

UniProt: G7NXR4

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation