BCAM Protein (AA 32-547) (His tag)

Catalog No. ABIN7273932

Product Details

Target: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Protein Type: Recombinant

Protein Characteristics: AA 32-547

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BCAM protein is labelled with His tag.

Purpose: Human BCAM Protein

Sequence: Glu32-Ala547

Characteristics: Recombinant Human BCAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu32-Ala547.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Alternative Name: BCAM (BCAM Products)

Background: Lutheran/basal cell adhesion molecule (Lu/BCAM) is a transmembrane adhesion molecule expressed by erythrocytes and endothelial cells that can interact with the extracellular matrix protein laminin-α5. In sickle cell disease, Lu/BCAM is thought to contribute to adhesion of sickle erythrocytes to the vascular wall, especially during vaso-occlusive crises.

Molecular Weight: 57.32 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

UniProt: P50895