VTCN1 Protein (AA 29-258) (Biotin,His-Avi Tag)

Catalog No. ABIN7273953

This Recombinant VTCN1 protein is produced in HEK-293 Cells.

Quick Overview for VTCN1 Protein (AA 29-258) (Biotin,His-Avi Tag) (ABIN7273953)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-258

Purification tag / Conjugate: This VTCN1 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human B7-H4 Protein

Sequence: Phe29-Ala258

Characteristics: Recombinant Biotinylated Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human B7-H4, His Tag at 2ug/ml (100ul/Well) on streptavidin(5ug/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).,Biotinylated Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-B7-H4 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human B7-H4, His-Avi Tag with an affinity constant of 6.90 nM as determined in BLI assay (Gator® Prime).,The purity of Biotinylated Human B7-H4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: B7-H4

Background: B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.

Molecular Weight: 28.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: Q7Z7D3