CD14 Protein (CD14) (AA 20-344) (His tag)
Quick Overview for CD14 Protein (CD14) (AA 20-344) (His tag) (ABIN7274064)
Target
See all CD14 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 20-344
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Purification tag / Conjugate
- This CD14 protein is labelled with His tag.
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Purpose
- Human CD14 Protein
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Sequence
- Thr20-Met344
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Characteristics
- Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Met344.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 20-352) protein (His tag)CD14 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-349) protein (His tag)CD14 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
CD14 (CD14) protein (His tag,Fc Tag)CD14 Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 Origin: Rat Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD14
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Alternative Name
- CD14
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Background
- Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities.
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Molecular Weight
- 38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
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Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
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