CD3 epsilon Protein (CD3E) (AA 22-48) (Fc-Avi Tag)

Catalog No. ABIN7274134

Product Details

Target: CD3 epsilon (CD3E)

Protein Type: Recombinant

Protein Characteristics: AA 22-48

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This CD3 epsilon protein is labelled with Fc-Avi Tag.

Purpose: Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein

Sequence: Asp22-Thr48

Characteristics: Recombinant Cynomolgus CD3E/CD3 epsilon 1-27 peptide Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp22-Thr48.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD3E 1-27 peptide, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 37.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E (CD3E Products)

Synonyms: cd3e Protein, CD3E Protein, t3e Protein, tcre Protein, AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, T3E Protein, TCRE Protein, CD3e molecule Protein, CD3 antigen, epsilon polypeptide Protein, cd3e Protein, CD3E Protein, Cd3e Protein

Background: CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Molecular Weight: 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q95LI5

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway