CD33 Protein (CD33) (AA 18-259) (His-Avi Tag)

Catalog No. ABIN7274159

Recombinant CD33 protein expressed in HEK-293 Cells.

Quick Overview for CD33 Protein (CD33) (AA 18-259) (His-Avi Tag) (ABIN7274159)

Target: CD33

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-259

Purification tag / Conjugate: This CD33 protein is labelled with His-Avi Tag.

Purpose: Human Siglec-3/CD33 Protein

Sequence: Asp18-His259

Characteristics: Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Asp18-His259.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0).

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0). Normally 8 % mannitol is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD33

Alternative Name: Siglec-3

Background: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.

Molecular Weight: 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

UniProt: P20138-1