CD83 Protein (CD83) (AA 20-147) (His tag)

Catalog No. ABIN7274246

Recombinant CD83 protein expressed in HEK-293 Cells.

Quick Overview for CD83 Protein (CD83) (AA 20-147) (His tag) (ABIN7274246)

Target: CD83

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-147

Purification tag / Conjugate: This CD83 protein is labelled with His tag.

Purpose: Cynomolgus CD83 Protein

Sequence: Thr20-Glu147

Characteristics: Recombinant Cynomolgus CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Glu147.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus CD83, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD83

Alternative Name: CD83

Background: CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Molecular Weight: 15.43 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5US85