SARS-CoV-2 Spike Protein (D614G, Trimer) (His-Avi Tag)

Catalog No. ABIN7274402

Product Details

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Protein Characteristics: D614G, Trimer

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.

Purpose: SARS-COV-2 Spike S Trimer (D614G) Protein

Sequence: Val16-Glu1188 (D614G)

Characteristics: Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 (D614G).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike S Trimer (D614G) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 46.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Products

Synonyms: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Background: The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.

Molecular Weight: 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.