DLL4 Protein (AA 27-524) (His-Avi Tag,Biotin)
-
- Target See all DLL4 Proteins
- DLL4 (delta-Like 4 (DLL4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-524
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This DLL4 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human DLL4 Protein
- Sequence
- Ser27-Pro524
- Characteristics
- Recombinant Biotinylated Human DLL4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser27-Pro524.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-DLL4 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC50 of 93.6ng/ml determined by ELISA. See testing image for detail.
-
delta-Like 4 (DLL4) (AA 27-524) protein (Fc Tag)
DLL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta-Like 4 (DLL4) (AA 558-685) protein (His-IF2DI Tag)DLL4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
delta-Like 4 (DLL4) (Active) protein (His tag)DLL4 Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- DLL4 (delta-Like 4 (DLL4))
- Alternative Name
- DLL4 (DLL4 Products)
- Background
- Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
- Molecular Weight
- 55.7 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9NR61
- Pathways
- Notch Signaling
-
