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FAP Protein (AA 26-760) (His-Avi Tag,Biotin)

Recombinant FAP protein expressed in HEK-293 Cells.
Catalog No. ABIN7274582

Quick Overview for FAP Protein (AA 26-760) (His-Avi Tag,Biotin) (ABIN7274582)

Target

See all FAP Proteins
FAP (Fibroblast Activation Protein, alpha (FAP))

Protein Type

Recombinant

Origin

  • 21
  • 8
  • 3
  • 2
Cynomolgus

Source

  • 19
  • 7
  • 2
  • 2
  • 2
  • 1
HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
  • Protein Characteristics

    AA 26-760

    Purification tag / Conjugate

    This FAP protein is labelled with His-Avi Tag,Biotin.

    Purpose

    Biotinylated Cynomolgus FAP Protein

    Sequence

    Leu26-Asp760

    Characteristics

    Recombinant Biotinylated Cynomolgus FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760.

    Sterility

    0.22 μm filtered

    Endotoxin Level

    Less than 1EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Anti-FAP Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus FAP, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail.
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  • Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Supplied as 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl, 200 mM Arginine ( pH 8.2).

    Storage

    -80 °C

    Storage Comment

    Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    FAP (Fibroblast Activation Protein, alpha (FAP))

    Alternative Name

    FAP

    Background

    Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

    Molecular Weight

    88 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

    NCBI Accession

    XP_005573377

    Pathways

    Tube Formation
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